Croote, D., Quake, S.R. Food allergen detection by mass spectrometry: the role of systems biology. npj Syst Biol Appl. 2016 Sep 29; 2:16022.
Peptide: YPILPEYLQCVK
Peptide within the protein Gal-d-2:
MGSIGAASMEFCFDVFKELKVHHANENIFYCPIAIMSALAMVYLGAKDSTRTQINKVVRFDKLPGFGDSIEAQCGTSVNVHSSLRDILNQITKPNDVYSFSLASRLYAEERYPILPEYLQCVKELYRGGLEPINFQTAADQARELINSWVESQTNGIIRNVLQPSSVDSQTAMVLVNAIVFKGLWEKAFKDEDTQAMPFRVTEQESKPVQMMYQIGLFRVASMASEKMKILELPFASGTMSMLVLLPDEVSGLEQLESIINFEKLTEWTSSNVMEERKIKVYLPRMKMEEKYNLTSVLMAMGITDVFSSSANLSGISSAESLKISQAVHAAHAEINEAGREVVGSAEAGVDAASVSEEFRADHPFLFCIKHIATNAVLFFGRCVSP
References reporting this peptide:
- Azarnia S., et al. Detection of ovalbumin in egg white, whole egg and incurred pasta using LC–ESI-MS/MS and ELISA. Food Research International. 2013
- Heick J., et al. Application of a liquid chromatography tandem mass spectrometry method for the simultaneous detection of seven allergenic foods in flour and bread and comparison of the method with commercially available ELISA test kits.. Journal of AOAC International. 2011
- Monaci L., et al. Multi-allergen quantification of fining-related egg and milk proteins in white wines by high-resolution mass spectrometry. Rapid Communications in Mass Spectrometry. 2013
- Monaci L., et al. Multi-allergen detection in food by micro high-performance liquid chromatography coupled to a dual cell linear ion trap mass spectrometry. Journal of Chromatography A. 2014
- Pilolli, et al. In house validation of a high resolution mass spectrometry Orbitrap-based method for multiple allergen detection in a processed model food. Analytical and Bioanalytical Chemistry. 2018
- Pilolli, et al. Streamlining the analytical workflow for multiplex MS/MS allergen detection in processed foods. Food Chemistry. 2017
Species Uniqueness
Species containing the peptide YPILPEYLQCVK are presented below. Accessions and taxid values link to further information hosted on NCBI.
| Species name | Common name | Accession(s) | Tax ID |
|---|---|---|---|
| Melopsittacus undulatus | Budgerigar | XP_005149989 |
13146 |
| Mesitornis unicolor | Brown roatelo | XP_010183742 |
54374 |
| Nestor notabilis | Kea | XP_010016792 |
176057 |
| Nipponia nippon | Crested ibis | XP_009473746 |
128390 |
| Opisthocomus hoazin | Opisthocomus hoazin | XP_009934773 |
30419 |
| Pelecanus crispus | Pelecanus crispus | XP_009490821 |
36300 |
| Phaethon lepturus | Phaethon lepturus | XP_010293973 |
97097 |
| Haliaeetus leucocephalus | Bald eagle | XP_010571604 |
52644 |
| Leptosomus discolor | Leptosomus discolor | XP_009959595 |
188344 |
| Meleagris gallopavo | Turkey | NP_001290119 XP_010706723 |
9103 |
| Acanthisitta chloris | Rifleman | XP_009072079 |
57068 |
| Amazona aestiva | Blue-fronted amazon | KQK83973 |
12930 |
| Apaloderma vittatum | Apaloderma vittatum | XP_009876650 |
57397 |
| Aquila chrysaetos canadensis | Aquila chrysaetos canadensis | XP_011579666 |
216574 |
| Balearica pavonina gibbericeps | Balearica pavonina gibbericeps | XP_010301139 |
100784 |
| Calypte anna | Anna's hummingbird | XP_008490821 |
9244 |
| Cariama cristata | Red-legged seriema | XP_009695563 |
54380 |
| Chlamydotis undulata macqueenii | Chlamydotis undulata macqueenii | XP_010123033 |
187382 |
| Cuculus canorus | Common cuckoo | XP_009567641 |
55661 |
| Fulmarus glacialis | Northern fulmar | XP_009580141 |
30455 |
| Gallus gallus | Chicken | 1UHG_A 1JTI_A 0705172A AAO43266 P01012 NP_990483 1OVA_B 1OVA_A 1OVA_C XP_015137652 |
9031 |
| Gavia stellata | Gavia stellata | XP_009809302 XP_009809301 |
37040 |
| Haliaeetus albicilla | White-tailed eagle | XP_009919470 |
8969 |
| Phalacrocorax carbo | Great cormorant | XP_009507610 XP_009507609 |
9209 |
| Philomachus pugnax | Philomachus pugnax | XP_014796284 |
198806 |
| Pterocles gutturalis | Yellow-throated sandgrouse | XP_010072413 |
240206 |
BLAST non-redundant protein database version: 4, date: December 9, 2015.
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