Rapidly develop targeted mass spectrometry assays using the consensus of published literature
This rose plot enables visualization of proteotypic peptides.
Each colored rose petal corresponds to a peptide and is bounded by thin gray petals, which represent tryptic cut sites.
The radial magnitude of each peptide corresponds to the number of publications in which is reported.
Therefore, peptides preferentially
reported in MS allergen detection literature stand out as radially large rose petals.
Hover over a rose petal with your mouse to see the peptide. Click on a rose petal to see the species
specificity of that peptide and add it to your cart.
Milk protein Bos d 10 (α-s2-casein)
From the above plot, 3 out of the 16 tryptic peptides within the 7 to 30 amino acid length range have clear preferential detection and use in published mass spectrometry studies. These peptides include:
Save peptides to your cart
In the upper right of every peptide page is a button to add a peptide to your cart. If you close
your browser, your cart should be preserved.
Example: add the above peptide ALNEINQFYQK to your cart by clicking below:
Next, you can view your cart:
Peptides in your cart can be easily exported in a comma separated value (csv) format that imports
into Skyline, an
open source, freely available software package for targeted proteomics. Once in Skyline, a targeted
method in native mass spectrometry instrument format can easily be exported.
Save a peptide to cart before exporting
Export as a csv
Less studied allergens
Insufficient published data for certain food allergens, such as tree nuts and wheat, implores further
research before a set of proteotypic peptides are established. To accelerate this effort, the
Allergen Peptide Browser includes tools for peptide selection, species specificity filtering, and
1. Select abundant allergenic proteins
A pie chart representing the relative abundances of the allergens in a food highlights those that
are most abundant. For some foods, this level of detail is unavailable and has been replaced with
estimates of protein family fractions. Clicking on a pie segment brings you to the page for that protein.
Allergenic milk proteins
2. Filter peptides by physiochemical properties
For each protein, potential peptide targets can be filtered by physiochemical properties. The table
can also be sorted by empirical count (Exp.), previous amino acid (1), subsequent amino acid (2),
or by in silico prediction scores by ESP Predictor (ESP) or CONSeQuence (CONSeQ).
Milk protein: Bos d 12 (κ-casein)
Click a checkbox to exclude:
3. Check species specificity
The table below depicts select results from the BLAST query of the non-redundant protein database
for the peptide YMVIQGEPGAVIR. Given that this
peptide is present in soybean, rice, and hazel proteins, it is not a suitable peptide for
specific allergen detection.
|Species name||Common name||Accession(s)||Tax id|
4. Save peptides and export
See left / above.