If you found this site useful, please cite: Croote, D., Quake, S.R. Food allergen detection by mass spectrometry: the role of systems biology. npj Syst Biol Appl. 2016 Sep 29; 2:16022.

Walnut Protein: Jug r 2 | Vicilin Seed Storage Protein

Empirical Proteotypic Peptide Explorer:

This rose plot enables visualization of proteotypic peptides. Each colored rose petal corresponds to a peptide and is bounded by thin gray petals, which represent tryptic cut sites. The radial magnitude of each peptide corresponds to the number of publications which report it.

Hover over a rose petal with your mouse to see the peptide. Click on a rose petal to see the species specificity of that peptide and add it to your cart.


Sequence - 593 amino acids

RGRDDDDEENPRDPREQYRQCQEYCRRQGQGQRQQQQCQIRCEERLEEDQRSQEERERRRGRDVDDQNPRDPEQRYEQCQQQCERQRRGQEQTLCRRRCEQRRQQEERERQRGRDRQDPQQQYHRCQRRCQIQEQSPERQRQCQQRCERQYKEQQGRERGPEASPRRESRGREEEQQRHNPYYFHSQSIRSRHESEEGEVKYLERFTERTELLRGIENYRVVILDANPNTSMLPHHKDAESVAVVTRGRATLTLVSQETRESFNLECGDVIRVPAGATVYVINQDSNERLEMVKLLQPVNNPGQFREYYAAGAKSPDQSYLRVFSNDILVAALNTPRDRLERFFDQQEQREGVIIRASQEKLRALSQHAMSAGQRPWGRRSSGGPISLKSESPSYSNQFGQFFEACPEEHRQLQEMDVLVNYAEIKRGAMMVPHYNSKATVVVYVVEGTGRYEMACPHVSSQSYEGQGRREQEEEESTGRFQKVTARLARGDIFVIPAGHPIAITASQNENLRLLGFDINGENNQRDFLAGQNNIINQLEREAKELSFNMPREEIEEIFESQMESYFVPTERQSRRGQGRDHPLASILDFAFF

UniProt: Q9SEW4   IUIS: Jug r 2

Peptide Selector Tool

Explore peptide targets for mass spectrometry by adjusting the selection criteria below. Results from empirical and computational prediction tools have been aggregated for convenience.

Exclude:
Peptide Characteristics:

Minimum length:

Maximum length:

1Peptide2Exp.3ESP4CONSeQ5
R DHPLASILDFAFF $ 0 0.687 0.13786
R GAMMVPHYNSK A 0 0.346 0.2431
R QDPQQQYHR C 0 0.248 0.24776
R QQQQCQIR C 0 0.214 0.3222
R DVDDQNPR D 0 0.219 0.13412
R DDDDEENPR D 0 0.254 0.14596
R VVILDANPNTSMLPHHK D 0 0.603 0.39462
R VFSNDILVAALNTPR D 2 0.818 0.64274
R LLGFDINGENNQR D 0 0.73 0.69558
K ELSFNMPR E 0 0.299 0.581
K LLQPVNNPGQFR E 1 0.791 0.74014
R FFDQQEQR E 1 0.307 0.3049
R DFLAGQNNIINQLER E 1 0.741 0.50704
R ATLTLVSQETR E 2 0.568 0.75268
R EQEEEESTGR F 0 0.233 0.17106
K DAESVAVVTR G 0 0.495 0.7259
R GDIFVIPAGHPIAITASQNENLR L 0 0.87 0.09846
R VPAGATVYVINQDSNER L 0 0.775 0.67612
K SESPSYSNQFGQFFEACPEEHR Q 0 0.586 0.38618
R EEIEEIFESQMESYFVPTER Q 0 0.482 0.21098
R YEQCQQQCER Q 0 0.303 0.28522
R CQIQEQSPER Q 0 0.305 0.34526
R GPEASPR R 0 0.167 0.1091
R QCQEYCR R 0 0.249 0.16538
R QLQEMDVLVNYAEIK R 0 0.554 0.27588
R YEMACPHVSSQSYEGQGR R 0 0.499 0.28242
R GQEQTLCR R 0 0.289 0.30244
R ALSQHAMSAGQRPWGR R 0 0.212 0.3027
R HNPYYFHSQSIR S 0 0.48 0.14444
R EYYAAGAK S 0 0.241 0.391
R SSGGPISLK S 0 0.271 0.4624
K SPDQSYLR V 0 0.308 0.38992
R ESFNLECGDVIR V 0 0.623 0.5159
K ATVVVYVVEGTGR Y 0 0.431 0.65836
R HESEEGEVK Y 0 0.241 0.13812

1 Previous amino acid (^ = Start of protein)

2 Next amino acid ($ = End of protein)

3 Exp. = Number of publications in which this peptide has been reported experimentally

4 ESP = ESP Predictor. Fusaro VA, et al. Nat Botechnol 2009; 27(2): 190-198. doi

5 CONSeQ = CONSeQuence. Eyers CE, et al. Mol Cell Proteomics 2011; 10(11). doi

Underline: Peptide occurs within the first 20 amino acids from the start of the protein. Use caution as the protein may contain a cleaved signaling sequence.

Strike-through: Peptide is present in a protein from another allergen species and is thus nonspecific.