If you found this site useful, please cite: Croote, D., Quake, S.R. Food allergen detection by mass spectrometry: the role of systems biology. npj Syst Biol Appl. 2016 Sep 29; 2:16022.

Walnut Protein: Jug r 4 | 11S Globulin Seed Storage Protein

Empirical Proteotypic Peptide Explorer:

This rose plot enables visualization of proteotypic peptides. Each colored rose petal corresponds to a peptide and is bounded by thin gray petals, which represent tryptic cut sites. The radial magnitude of each peptide corresponds to the number of publications which report it.

Hover over a rose petal with your mouse to see the peptide. Click on a rose petal to see the species specificity of that peptide and add it to your cart.


Sequence - 507 amino acids

MAKPILLSIYLFLIVALFNGCLAQSGGRQQQQFGQCQLNRLDALEPTNRIEAEAGVIESWDPNNQQFQCAGVAVVRRTIEPNGLLLPQYSNAPQLVYIARGRGITGVLFPGCPETFEESQRQSQQGQSREFQQDRHQKIRHFREGDIIAFPAGVAHWSYNDGSNPVVAISLLDTNNNANQLDQNPRNFYLAGNPDDEFRPQGQQEYEQHRRQQQRQQRPGEHGQQQRGLGNNVFSGFDADFLADAFNVDTETARRLQSENDHRRSIVRVEGRQLQVIRPRWSREEQEREERKERERERESESERRQSRRGGRDDNGLEETICTLRLRENIGDPSRADIYTEEAGRISTVNSHTLPVLRWLQLSAERGALYSDALYVPHWNLNAHSVVYALRGRAEVQVVDNFGQTVFDDELREGQLLTIPQNFAVVKRARNEGFEWVSFKTNENAMVSPLAGRTSAIRALPEEVLATAFQIPREDARRLKFNRQESTLVRSRPSRSRSSRSERRAEV

UniProt: Q2TPW5   IUIS: Jug r 4

Peptide Selector Tool

Explore peptide targets for mass spectrometry by adjusting the selection criteria below. Results from empirical and computational prediction tools have been aggregated for convenience.

Exclude:
Peptide Characteristics:

Minimum length:

Maximum length:

1Peptide2Exp.3ESP4CONSeQ5
R ENIGDPSR A 0 0.19 0.17608
R AEVQVVDNFGQTVFDDELR E 0 0.61 0.09684
R QSQQGQSR E 0 0.235 0.17136
R ALPEEVLATAFQIPR E 0 0.894 0.7228
R GALYSDALYVPHWNLNAHSVVYALR G 0 0.384 0.03904
R TIEPNGLLLPQYSNAPQLVYIAR G 0 0.767 0.06156
R QQRPGEHGQQQR G 0 0.189 0.22354
R WLQLSAER G 0 0.304 0.40794
R ADIYTEEAGR I 1 0.416 0.77404
R LDALEPTNR I 2 0.481 0.84376
R QQQQFGQCQLNR L 0 0.418 0.37494
R DDNGLEETICTLR L 0 0.685 0.59636
R GITGVLFPGCPETFEESQR Q 0 0.848 0.22976
^ MAKPILLSIYLFLIVALFNGCLAQSGGR Q 0 0.192 0.04794
R GLGNNVFSGFDADFLADAFNVDTETAR R 0 0.411 0.0563
R IEAEAGVIESWDPNNQQFQCAGVAVVR R 0 0.389 0.0816
R LQSENDHR R 0 0.279 0.13924
R NFYLAGNPDDEFRPQGQQEYEQHR R 0 0.436 0.38304
R EGQLLTIPQNFAVVK R 0 0.779 0.69952
R QESTLVR S 0 0.26 0.2324
K TNENAMVSPLAGR T 0 0.669 0.95078
R NEGFEWVSFK T 0 0.498 0.27626
R QLQVIRPR W 0 0.224 0.43562
R ISTVNSHTLPVLR W 0 0.725 0.67902

1 Previous amino acid (^ = Start of protein)

2 Next amino acid ($ = End of protein)

3 Exp. = Number of publications in which this peptide has been reported experimentally

4 ESP = ESP Predictor. Fusaro VA, et al. Nat Botechnol 2009; 27(2): 190-198. doi

5 CONSeQ = CONSeQuence. Eyers CE, et al. Mol Cell Proteomics 2011; 10(11). doi

Underline: Peptide occurs within the first 20 amino acids from the start of the protein. Use caution as the protein may contain a cleaved signaling sequence.

Strike-through: Peptide is present in a protein from another allergen species and is thus nonspecific.