If you found this site useful, please cite: Croote, D., Quake, S.R. Food allergen detection by mass spectrometry: the role of systems biology. npj Syst Biol Appl. 2016 Sep 29; 2:16022.

Allergen Targets

Milk


Food Matrix

Dairy milk

Development of an SI-Traceable HPLC–Isotope Dilution Mass Spectrometry Method To Quantify β-Lactoglobulin in Milk Powders

Yang W., Liqing W., Fei D., Bin Y., Yi Y., Jing W.

Journal of Agricultural and Food Chemistry (2014), 62, 14, 3073--3080 DOI: 10.1021/jf4054337

Abstract

β-Lactoglobulin (β-LG) is one of the major allergenic proteins in milk. There is an urgent demand for an accurate and traceable method to develop β-LG certified reference material (CRM). In this work, β-LG was enzymatically digested and a specific peptide was chosen for quantitation by isotope-dilution mass spectrometry (IDMS). With amino acid CRMs as standards, the results could be traced to SI unit. By the proposed method, the recovery ranged from 86.0% to 118.3% with CVs <9.0%. The LOD and LOQ were 4.8 x 10^-5 g/g and 1.6 x 10^-4 g/g of β-LG in milk powder, respectively. Ten samples from domestic market were analyzed with CVs <5.6%, and the relative expanded uncertainties ranged from 4.2% to 5.9% (k = 2). With the CRMs, it is expected that the comparability of β-LG quantitation results will be improved among different laboratories.