Allergen Targets

Milk

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Food Matrices

Dairy milk

Snacks

Detection of the major milk allergen alpha-casein in food with liquid chromatography-tandem mass spectrometry in multiple reaction monitoring mode

Feng X., Zhang J., Lü M., Gao M., Zhang X.

Se pu = Chinese journal of chromatography / Zhongguo hua xue hui (2013), 31, 6, 510--513 DOI: 10.3724/SP.J.1123.2013.01003

Abstract

A new method of detecting milk allergen protein in food was established. Using multiple reaction monitoring (MRM) mode in high performance liquid chromatography-mass spectrometry (HPLC-MS) to detect milk allergen protein α-casein is successful and convenient. All transitions gave a linear response in the chosen concentration range of the milk allergen protein (between 0. 5 and 250 mg/L total soluble protein, 5 data points), and the detection limit is 0.5 mg/L of α-casein. Food samples from markets were tested. The results showed that MRM method could be used to detect the milk allergen in food products.

Reference raw data

Reference ID	Food	Protein	Peptide
37	Milk	Bos d 9	FFVAPFPEVFGK
37	Milk	Bos d 10	NAVPITPTLNR
37	Milk	Bos d 9	YLGYLEQLLR