Allergen Targets

Egg

Milk

Peanut

Soy

Tree nuts

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Food Matrices

Chocolate

Ice cream

Cookies

Sauce

Liquid chromatography coupled to tandem mass spectrometry for detecting ten allergens in complex and incurred foodstuffs

Planque, M., Arnould, T., Dieu, M., Delahaut, P., Renard, P., Gillard, N.

Journal of Chromatography A (2017), 1530, 138--151 DOI: 10.1016/J.CHROMA.2017.11.039

Abstract

Food allergy is a considerable heath problem, as undesirable contaminations by allergens during food production are still widespread and may be dangerous for human health. To protect the population, laboratories need to develop reliable analytical methods in order to detect allergens in various food products. Currently, a large majority of allergen-related food recalls concern bakery products. It is therefore essential to detect allergens in unprocessed and processed foodstuffs. In this study, we developed a method for detecting ten allergens in complex (chocolate, ice cream) and processed (cookie, sauce) foodstuffs, based on ultra-high performance liquid chromatography coupled to tandem mass spectrometry (UHPLC–MS/MS). Using a single protocol and considering a signal-to-noise ratio higher than 10 for the most abundant multiple reaction monitoring (MRM) transition, we were able to detect target allergens at 0.5mg/kg for milk proteins, 2.5mg/kg for peanut, hazelnut, pistachio, and cashew proteins, 3mg/kg for egg proteins, and 5mg/kg for soy, almond, walnut, and pecan proteins. The ability of the method to detect 10 allergens with a single protocol in complex and incurred food products makes it an attractive alternative to the ELISA method for routine laboratories.

Reference raw data

Reference ID	Food	Protein	Peptide
79	Hazelnut	Cor a 9	ADIYTEQVGR
79	Cashew	Ana o 2	ADIYTPEVGR
79	Hazelnut	Cor a 9	ALPDDVLANAFQISR
79	Almond	Pru du 6	ALPDEVLANAYQISR
79	Pistachio	Pis v 2	ALPLDVIK
79	Pistachio	Pis v 5	AMISPLAGSTSVLR
79	Cashew	Ana o 2	AMTSPLAGR
79	Pecan	Car i 2	ATLTFVSQER
79	Walnut	Jug r 2	ATLTLVSQETR
79	Cashew	Ana o 2	CAGVALVR
79	Soy	Gly m 6	EAFGVNMQIVR
79	Soy	Gly m 8	ELINLATMCR
79	Cashew	Ana o 3	ELYETASELPR
79	Milk	Bos d 9	FFVAPFPEVFGK
79	Peanut	Ara h 3	FNLAGNHEQEFLR
79	Walnut	Jug r 1	GEEMEEMVQSAR
79	Egg	Gal d 2	GGLEPINFQTAADQAR
79	Almond	Pru du 6	GNLDFVQPPR
79	Milk	Bos d 9	HQGLPQEVLNENLLR
79	Hazelnut	Cor a 9	INTVNSNTLPVLR
79	Egg	Gal d 2	ISQAVHAAHAEINEAGR
79	Soy	Gly m 6	ISTLNSLTLPALR
79	Pistachio	Pis v 5	ITSLNSLNLPILK
79	Soy	Gly m 5	LITLAIPVNKPGR
79	Walnut	Jug r 2	LLQPVNNPGQFR
79	Hazelnut	Cor a 9	LNALEPTNR
79	Pistachio	Pis v 1	LQELYETASELPR
79	Milk	Bos d 5	LSFNPTQLEEQCHI
79	Egg	Gal d 2	LTEWTSSNVMEER
79	Pecan	Car i 2	LVGFGINGK
79	Milk	Bos d 10	NAVPITPTLNR
79	Pecan	Car i 2	NFLAGQNNIINQLER
79	Peanut	Ara h 2	NLPQQCGLR
79	Almond	Pru du 6	QETIALSSSQQR
79	Hazelnut	Cor a 9	QGQVLTIPQNFAVAK
79	Pecan	Car i 2	QVESYFVPMER
79	Peanut	Ara h 3	RPFYSNAPQEIFIQQGR
79	Almond	Pru du 6	TDENGFTNTLAGR
79	Hazelnut	Cor a 9	TNDNAQISPLAGR
79	Soy	Gly m 6	VFDGELQEGR
79	Walnut	Jug r 2	VFSNDILVAALNTPR
79	Milk	Bos d 5	VLVLDTDYK
79	Pistachio	Pis v 2	VTSINALNLPILR
79	Milk	Bos d 5	VYVEELKPTPEGDLEILLQK
79	Milk	Bos d 9	YLGYLEQLLR
79	Egg	Gal d 6	YLLDLLPAAASHR
79	Almond	Pru du 6	YNRQETIALSSSQQR