Allergen Targets

Egg

Milk

Peanut

Soy

Tree nuts

---

Food Matrices

Chocolate bar

Broth powder

Discovery based high resolution MS/MS analysis for selection of allergen markers in chocolate and broth powder matrices

Pilolli, R., Van Poucke, C., De Angelis, E., Nitride, C., de Loose, M., Gillard, N., Huet, A.-C., Tranquet, O., Laré, C., Adel-Patient, K., Bernard, H., Mills, E.N.C. and Monaci, L.

Food Chemistry (2021), 343, 128533 DOI: 10.1016/j.foodchem.2020.128533

Abstract

Peptide marker identification is an important step in development of a mass spectrometry method for multiple allergen detection, since specificity, robustness and sensitivity of the overall analytical method will depend on the reliability of the proteotypic peptides. As part of the development of a multi-analyte reference method, discovery analysis of two incurred food matrices has been undertaken to select the most reliable peptide markers. Six allergenic ingredients (milk, egg, peanut, soybean, hazelnut, and almond) were incurred into either chocolate or broth powder matrix. Different conditions of protein extraction and purification were tested and the tryptic peptide pools were analysed by untargeted high resolution tandem mass spectrometry and the resulting fragmentation spectra were processed via a commercial software for sequence identification. The analysis performed on incurred foods provides both a prototype effective and straightforward sample preparation protocol and delivers reliable peptides to be included in a standardized selected reaction monitoring method.

Reference raw data

Reference ID	Food	Protein	Peptide
89	Almond	Pru du 6	ADIFSPR
89	Hazelnut	Cor a 9	ALPDDVLANAFQISR
89	Almond	Pru du 6	ALPDEVLANAYQISR
89	Egg	Gal d 6	ATAVSLLEWQR
89	Milk	Bos d 10	FALPQYLK
89	Milk	Bos d 9	FFVAPFPEVFGK
89	Almond	Pru du 6	FYLAGNPENEFNQQGQSQPR
89	Soy	Gly m 6	FYLAGNQEQEFLK
89	Egg	Gal d 2	GGLEPINFQTAADQAR
89	Almond	Pru du 6	GNLDFVQPPR
89	Milk	Bos d 9	HQGLPQEVLNENLLR
89	Egg	Gal d 2	ISQAVHAAHAEINEAGR
89	Almond	Pru du 6	ISTLNSHNLPILR
89	Soy	Gly m 6	ISTLNSLTLPALR
89	Peanut	Ara h 3	TANDLNLLILR
89	Almond	Pru du 6	TEENAFINTLAGR
89	Soy	Gly m 6	VLIVPQNFVVAAR
89	Milk	Bos d 11	VLPVPQK
89	Milk	Bos d 5	VYVEELKPTPEGDLEILLQK
89	Milk	Bos d 12	YIPIQYVLSR
89	Milk	Bos d 9	YLGYLEQLLR