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Croote, D., Quake, S.R. Food allergen detection by mass spectrometry: the role of systems biology. npj Syst Biol Appl. 2016 Sep 29; 2:16022.

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Peanut Protein: Ara h 1 | Cupin (Vicillin-Type, 7S Globulin)

Empirical Proteotypic Peptide Explorer:

This rose plot enables visualization of proteotypic peptides. Each colored rose petal corresponds to a peptide and is bounded by thin gray petals, which represent tryptic cut sites. The radial magnitude of each peptide corresponds to the number of publications which report it.

Hover over a rose petal with your mouse to see the peptide. Click on a rose petal to see the species specificity of that peptide and add it to your cart.


Sequence - 626 amino acids

MRGRVSPLMLLLGILVLASVSATHAKSSPYQKKTENPCAQRCLQSCQQEPDDLKQKACESRCTKLEYDPRCVYDPRGHTGTTNQRSPPGERTRGRQPGDYDDDRRQPRREEGGRWGPAGPREREREEDWRQPREDWRRPSHQQPRKIRPEGREGEQEWGTPGSHVREETSRNNPFYFPSRRFSTRYGNQNGRIRVLQRFDQRSRQFQNLQNHRIVQIEAKPNTLVLPKHADADNILVIQQGQATVTVANGNNRKSFNLDEGHALRIPSGFISYILNRHDNQNLRVAKISMPVNTPGQFEDFFPASSRDQSSYLQGFSRNTLEAAFNAEFNEIRRVLLEENAGGEQEERGQRRWSTRSSENNEGVIVKVSKEHVEELTKHAKSVSKKGSEEEGDITNPINLREGEPDLSNNFGKLFEVKPDKKNPQLQDLDMMLTCVEIKEGALMLPHFNSKAMVIVVVNKGTGNLELVAVRKEQQQRGRREEEEDEDEEEEGSNREVRRYTARLKEGDVFIMPAAHPVAINASSELHLLGFGINAENNHRIFLAGDKDNVIDQIEKQAKDLAFPGSGEQVEKLIKNQKESHFVSARPQSQSQSPSSPEKESPEKEDQEEENQGGKGPLLSILKAFN

UniProt: P43238   IUIS: Ara h 1

Peptide Selector Tool

Explore peptide targets for mass spectrometry by adjusting the selection criteria below. Results from empirical and computational prediction tools have been aggregated for convenience.

Exclude:
Peptide Characteristics:

Minimum length:

Maximum length:

1 Peptide 2 Exp.3 ESP4 CONSeQ5
R VSPLMLLLGILVLASVSATHAK S 0 0.444 0.12606
K TENPCAQR C 0 0.262 0.19602
R CLQSCQQEPDDLK Q 0 0.567 0.36178
R GHTGTTNQR S 0 0.331 0.1523
R QPGDYDDDR R 0 0.261 0.16496
R WGPAGPR E 0 0.262 0.22094
R RPSHQQPR K 0 0.182 0.19222
R EGEQEWGTPGSHVR E 1 0.608 0.39554
R NNPFYFPSR R 4 0.489 0.43444
R YGNQNGR I 0 0.25 0.11132
R QFQNLQNHR I 1 0.366 0.23524
R IVQIEAKPNTLVLPK H 1 0.433 0.7547
K HADADNILVIQQGQATVTVANGNNR K 0 0.669 0.31584
K SFNLDEGHALR I 4 0.713 0.56688
R IPSGFISYILNR H 0 0.638 0.3591
R HDNQNLR V 0 0.25 0.11876
K ISMPVNTPGQFEDFFPASSR D 1 0.911 0.17808
R DQSSYLQGFSR N 1 0.597 0.44536
R NTLEAAFNAEFNEIR R 3 0.7 0.47562
R VLLEENAGGEQEER G 6 0.618 0.70694
R SSENNEGVIVK V 1 0.504 0.62688
K EHVEELTK H 1 0.278 0.27542
K GSEEEGDITNPINLR E 0 0.729 0.82228
R EGEPDLSNNFGK L 1 0.549 0.33306
K LFEVKPDK K 0 0.251 0.56546
K NPQLQDLDMMLTCVEIK E 0 0.556 0.12114
K EGALMLPHFNSK A 1 0.544 0.54662
K AMVIVVVNK G 2 0.312 0.36754
K GTGNLELVAVR K 6 0.658 0.85124
R EEEEDEDEEEEGSNR E 1 0.39 0.2348
R IFLAGDK D 0 0.263 0.60074
K DNVIDQIEK Q 0 0.345 0.47952
K DLAFPGSGEQVEK L 6 0.65 0.92054
K ESHFVSARPQSQSQSPSSPEK E 0 0.289 0.3648
K EDQEEENQGGK G 0 0.248 0.16262
K GPLLSILK A 0 0.31 0.55638

1 Previous amino acid (^ = Start of protein)

2 Next amino acid ($ = End of protein)

3 Exp. = Number of publications in which this peptide has been reported experimentally

4 ESP = ESP Predictor. Fusaro VA, et al. Nat Botechnol 2009; 27(2): 190-198. doi

5 CONSeQ = CONSeQuence. Eyers CE, et al. Mol Cell Proteomics 2011; 10(11). doi

Underline: Peptide occurs within the first 20 amino acids from the start of the protein. Use caution as the protein may contain a cleaved signaling sequence.

Strike-through: Peptide is present in a protein from another allergen species and is thus nonspecific.

 

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