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Croote, D., Quake, S.R. Food allergen detection by mass spectrometry: the role of systems biology. npj Syst Biol Appl. 2016 Sep 29; 2:16022.

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  3. Ara h 3

Peanut Protein: Ara h 3 | Cupin (Legumin-Type, 11S Globulin, Glycinin)

Isoform:

Empirical Proteotypic Peptide Explorer:

This rose plot enables visualization of proteotypic peptides. Each colored rose petal corresponds to a peptide and is bounded by thin gray petals, which represent tryptic cut sites. The radial magnitude of each peptide corresponds to the number of publications which report it.

Hover over a rose petal with your mouse to see the peptide. Click on a rose petal to see the species specificity of that peptide and add it to your cart.


Sequence - 507 amino acids

RQQPEENACQFQRLNAQRPDNRIESEGGYIETWNPNNQEFECAGVALSRLVLRRNALRRPFYSNAPQEIFIQQGRGYFGLIFPGCPRHYEEPHTQGRRSQSQRPPRRLQGEDQSQQQRDSHQKVHRFDEGDLIAVPTGVAFWLYNDHDTDVVAVSLTDTNNNDNQLDQFPRRFNLAGNTEQEFLRYQQQSRQSRRRSLPYSPYSPQSQPRQEEREFSPRGQHSRRERAGQEEENEGGNIFSGFTPEFLEQAFQVDDRQIVQNLRGETESEEEGAIVTVRGGLRILSPDRKRRADEEEEYDEDEYEYDEEDRRRGRGSRGRGNGIEETICTASAKKNIGRNRSPDIYNPQAGSLKTANDLNLLILRWLGPSAEYGNLYRNALFVAHYNTNAHSIIYRLRGRAHVQVVDSNGNRVYDEELQEGHVLVVPQNFAVAGKSQSENFEYVAFKTDSRPSIANLAGENSVIDNLPEEVVANSYGLQREQARQLKNNNPFKFFVPPSQQSPRAVA

UniProt: O82580   IUIS: Ara h 3

Empirical Proteotypic Peptide Explorer:

This rose plot enables visualization of proteotypic peptides. Each colored rose petal corresponds to a peptide and is bounded by thin gray petals, which represent tryptic cut sites. The radial magnitude of each peptide corresponds to the number of publications which report it.

Hover over a rose petal with your mouse to see the peptide. Click on a rose petal to see the species specificity of that peptide and add it to your cart.


Sequence - 530 amino acids

MAKLLELSFCFCFLVLGASSISFRQQPEENACQFQRLNAQRPDNRIESEGGYIETWNPNNQEFECAGVALSRLVLRRNALRRPFYSNAPQEIFIQQGRGYFGLIFPGCPSTYEEPAQQGRRYQSQRPPRRLQEEDQSQQQQDSHQKVHRFNEGDLIAVPTGVAFWLYNDHDTDVVAVSLTDTNNNDNQLDQFPRRFNLAGNHEQEFLRYQQQSRQSRRRSLPYSPYSPHSRPRREEREFRPRGQHSRRERAGQEEEDEGGNIFSGFTPEFLEQAFQVDDRQIVQNLWGENESEEEGAIVTVRGGLRILSPDGTRGADEEEEYDEDQYEYHEQDGRRGRGSRGGGNGIEETICTACVKKNIGGNRSPHIYDPQRWFTQNCHDLNLLILRWLGLSAEYGNLYRNALFVPHYNTNAHSIIYALRGRAHVQVVDSNGNRVYDEELQEGHVLVVPQNFAVAGKSQSENFEYVAFKTDSRPSIANFAGENSFIDNLPEEVVANSYGLPREQARQLKNNNPFKFFVPPFQQSPRAVA

UniProt: Q9SQH7   IUIS: Ara h 3

Peptide Selector Tool

Explore peptide targets for mass spectrometry by adjusting the selection criteria below. Results from empirical and computational prediction tools have been aggregated for convenience.

Exclude:
Peptide Characteristics:

Minimum length:

Maximum length:

1Peptide2Exp.3ESP4CONSeQ5
R QQPEENACQFQR L 2 0.43 0.38616
R LNAQRPDNR I 3 0.239 0.1893
R IESEGGYIETWNPNNQEFECAGVALSR L 0 0.386 0.10116
R RPFYSNAPQEIFIQQGR G 7 0.352 0.34374
R GYFGLIFPGCPR H 0 0.638 0.3769
R HYEEPHTQGR R 0 0.365 0.14644
R SQSQRPPR R 0 0.207 0.1644
R LQGEDQSQQQR D 0 0.32 0.28052
R FNLAGNTEQEFLR Y 0 0.736 0.58388
R SLPYSPYSPQSQPR Q 0 0.685 0.38706
R AGQEEENEGGNIFSGFTPEFLEQAFQVDDR Q 0 0.476 0.31282
R QIVQNLR G 2 0.24 0.37028
R GETESEEEGAIVTVR G 2 0.567 0.63084
R ADEEEEYDEDEYEYDEEDR R 1 0.226 0.3006
R GNGIEETICTASAK K 0 0.577 0.72198
R SPDIYNPQAGSLK T 10 0.693 0.78552
K TANDLNLLILR W 2 0.665 0.42998
R WLGPSAEYGNLYR N 0 0.641 0.3896
R NALFVAHYNTNAHSIIYR L 0 0.486 0.11108
R AHVQVVDSNGNR V 3 0.674 0.39236
R VYDEELQEGHVLVVPQNFAVAGK S 1 0.679 0.08226
K SQSENFEYVAFK T 3 0.626 0.47618
K FFVPPSQQSPR A 1 0.648 0.55052
K LLELSFCFCFLVLGASSISFR Q 0 0.164 0.05036
R QQPEENACQFQR L 2 0.43 0.38616
R LNAQRPDNR I 3 0.239 0.1893
R IESEGGYIETWNPNNQEFECAGVALSR L 0 0.386 0.10116
R RPFYSNAPQEIFIQQGR G 7 0.352 0.34374
R GYFGLIFPGCPSTYEEPAQQGR R 1 0.746 0.17966
R YQSQRPPR R 0 0.227 0.2323
R LQEEDQSQQQQDSHQK V 0 0.473 0.33526
R FNLAGNHEQEFLR Y 6 0.679 0.38002
R SLPYSPYSPHSRPR R 0 0.25 0.28026
R AGQEEEDEGGNIFSGFTPEFLEQAFQVDDR Q 0 0.47 0.31752
R QIVQNLWGENESEEEGAIVTVR G 1 0.429 0.11842
R ILSPDGTR G 0 0.282 0.731
R GADEEEEYDEDQYEYHEQDGR R 0 0.319 0.29374
R GGGNGIEETICTACVK K 0 0.571 0.50222
R SPHIYDPQR W 0 0.336 0.31046
R WFTQNCHDLNLLILR W 0 0.435 0.0559
R WLGLSAEYGNLYR N 3 0.527 0.2473
R NALFVPHYNTNAHSIIYALR G 1 0.483 0.08724
R AHVQVVDSNGNR V 3 0.674 0.39236
R VYDEELQEGHVLVVPQNFAVAGK S 1 0.679 0.08226
K SQSENFEYVAFK T 3 0.626 0.47618
K FFVPPFQQSPR A 1 0.599 0.57298

1 Previous amino acid (^ = Start of protein)

2 Next amino acid ($ = End of protein)

3 Exp. = Number of publications in which this peptide has been reported experimentally

4 ESP = ESP Predictor. Fusaro VA, et al. Nat Botechnol 2009; 27(2): 190-198. doi

5 CONSeQ = CONSeQuence. Eyers CE, et al. Mol Cell Proteomics 2011; 10(11). doi

Underline: Peptide occurs within the first 20 amino acids from the start of the protein. Use caution as the protein may contain a cleaved signaling sequence.

Strike-through: Peptide is present in a protein from another allergen species and is thus nonspecific.

 

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