Croote, D., Quake, S.R. Food allergen detection by mass spectrometry: the role of systems biology. npj Syst Biol Appl. 2016 Sep 29; 2:16022.
Peptide: DLAFPGSGEQVEK
Peptide within the protein Ara-h-1:
MRGRVSPLMLLLGILVLASVSATHAKSSPYQKKTENPCAQRCLQSCQQEPDDLKQKACESRCTKLEYDPRCVYDPRGHTGTTNQRSPPGERTRGRQPGDYDDDRRQPRREEGGRWGPAGPREREREEDWRQPREDWRRPSHQQPRKIRPEGREGEQEWGTPGSHVREETSRNNPFYFPSRRFSTRYGNQNGRIRVLQRFDQRSRQFQNLQNHRIVQIEAKPNTLVLPKHADADNILVIQQGQATVTVANGNNRKSFNLDEGHALRIPSGFISYILNRHDNQNLRVAKISMPVNTPGQFEDFFPASSRDQSSYLQGFSRNTLEAAFNAEFNEIRRVLLEENAGGEQEERGQRRWSTRSSENNEGVIVKVSKEHVEELTKHAKSVSKKGSEEEGDITNPINLREGEPDLSNNFGKLFEVKPDKKNPQLQDLDMMLTCVEIKEGALMLPHFNSKAMVIVVVNKGTGNLELVAVRKEQQQRGRREEEEDEDEEEEGSNREVRRYTARLKEGDVFIMPAAHPVAINASSELHLLGFGINAENNHRIFLAGDKDNVIDQIEKQAKDLAFPGSGEQVEKLIKNQKESHFVSARPQSQSQSPSSPEKESPEKEDQEEENQGGKGPLLSILKAFN
References reporting this peptide:
- Chassaigne H., et al. Proteomics-Based Approach To Detect and Identify Major Allergens in Processed Peanuts by Capillary LC-Q-TOF (MS/MS). Journal of Agricultural and Food Chemistry. 2007
- Chassaigne H., et al. Resolution and identification of major peanut allergens using a combination of fluorescence two-dimensional differential gel electrophoresis, Western blotting and Q-TOF mass spectrometry. Journal of Proteomics. 2009
- Heick J., et al. Application of a liquid chromatography tandem mass spectrometry method for the simultaneous detection of seven allergenic foods in flour and bread and comparison of the method with commercially available ELISA test kits.. Journal of AOAC International. 2011
- Pedreschi R., et al. Current Challenges in Detecting Food Allergens by Shotgun and Targeted Proteomic Approaches: A Case Study on Traces of Peanut Allergens in Baked Cookies. Nutrients. 2012
- Sayers, et al. Microfluidic separation coupled to mass spectrometry for quantification of peanut allergens in a complex food matrix. Journal of Proteome Research. 2017
- Shefcheck K.J., et al. Confirmation of Peanut Protein Using Peptide Markers in Dark Chocolate Using Liquid Chromatography−Tandem Mass Spectrometry (LC-MS/MS). Journal of Agricultural and Food Chemistry. 2006
Species Uniqueness
Species containing the peptide DLAFPGSGEQVEK are presented below. Accessions and taxid values link to further information hosted on NCBI.
| Species name | Common name | Accession(s) | Tax ID |
|---|---|---|---|
| Arachis duranensis | Peanut ancestor | CCW28760 XP_015941445 |
130453 |
| Arachis hypogaea | Peanut | ADV58148 AAT00595 AAT00594 3SMH_A 3S7E_A AAT00596 AAT00597 P43237 ADQ53858 P43238 |
3818 |
| Arachis ipaensis | Arachis ipaensis | XP_016174800 |
130454 |
BLAST non-redundant protein database version: 4, date: December 9, 2015.
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